In this study, it was aimed to produce Fe3O4 magnetic nanoparticles from the Euphorbia plant (Euphorbia amygdaloides) using a simple and economical green biosynthesis method as a new method. Iron oxide nanoparticles have a large number of applications (health, drug development, biosensor, imaging systems etc.) One of these Fe3O4 magnetic nanoparticles (MNPs) has been found to be used repeatedly in various application areas due to their properties. Fe3O4 MNPs were synthesized using euphorbia (Euphorbia amygdaloides) plant extract and Fe+2/+3 solution. The characterization analysis was performed using UV-Vis spectrophotometer, SEM, XRD and FTIR devices. Then, the mimetic (enzyme-like activity) properties of MNPs were investigated. It was determined that Fe3O4 MNPs mimics enzymes. They have similar properties and catalysis power to natural peroxidases. In the targeted new method, glucose oxidase (GOx) enzyme with Fe3O4 MNPs which mimic peroxidase have been used for developing a spectrophotometric method for highly sensitive and stable - enzymatic glucose determination in different foods. The method developed was found to be linear in the 0.25-1 g/L glucose range. The optimal operating parameters of the biosensor were found temperature, (45 oC), pH (4.0), metal ion concentration (5 mM) and H2O2 concentration (12.5 Mm), respectively. In addition, this method has shown a high selectivity to glucose compared to other sugars such as sucrose, lactose, fructose and raffinose.
Sümeyra Gunduz, Hayrunnisa Nadaroglu, Azize Alayli. Development of a new method for the determination of glucose in foods using an enzyme-like activity of Fe3O4 magnetic nanoparticles. International Journal of Research in Advanced Engineering and Technology, Volume 5, Issue 3, 2019, Pages 42-45